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Time course for synthesis of hexyl-glucoside (−∙−) and p-nitrophenol (…) from p-nitrophenol-d-glucoside (—) catalyzed by TmBglA (▲), N222F (△), Y295F (◊) and F414S (○). Reaction conditions: 34 mM p-nitrophenol-d-glucoside and 80% hexanol (vol/vol), 10 μg/ml purified enzyme, 60°C.
