Table 1.
Mutagenic Primer Sequences
| Primer | Mutation | Direction | Sequence(5′–3′) |
|---|---|---|---|
| 1 | N222F | Forward | AGTTTTCTTCAATGGATATTTCGAACCTGC |
| Reverse | ATTCCGATCTTTCCATCTTTCACG | ||
| 2 | N223C | Forward | AGTTTTCAATTGCGGATATTTCGAACCTGCG |
| Reverse | ATTCCGATCTTTCCATCTTTCACG | ||
| 3 | N223Q | Forward | AGTTTTCAATCAGGGATATTTCGAACCTGCG |
| Reverse | ATTCCGATCTTTCCATCTTTCACG | ||
| 4 | G224A | Forward | AGTATTCAACAACGCGTATTTCGAACCTGCGAGT |
| Reverse | ATTCCGATCTTTCCATCTTTCACG | ||
| 5 | Y295F | Forward | AACTATTTTTCCGGTCATTTGGTGAAG |
| Reverse | CAATCCAACAAAGTCGATCTTTTC | ||
| 6 | F414S | Forward | AGATCTGGTATTGTGTATGTAGACTAC |
| Reverse | CTTGGAATATCCCTCTGCCCATTC |
Note. Bold residues indicate the sites of mutation.