Figure 6.

SR-A neutralizing antibody attenuates osteoclastogenesis in CIA mice ex vivo. BMMs from CIA mice treated with SR-A neutralizing antibody or isotype antibody as described in Figure 5 were harvested for osteoclast differentiation ex vivo. After 9 days, the cells were fixed and set for TRAP staining, and multinucleated cells (more than three nuclei) with TRAP positivity were counted. The typical figures and the statistical result were shown, respectively (A). Scale bar = 50 μm. The relative expression of CTSK, TRAP, and MMP-9 was also evaluated by qPCR (B). The statistical results are presented as mean ± SEM (two-tailed paired Student’ s t test, ∗P < 0.05, ∗∗P < 0.01).