Table 1.

Overview of 13R-MO, 13R-MO Derivatives, and Forskolin Studies in Biosynthesis

Host	Key pathways	Key enzymes	Product	Titer	References
E. coli	MEP	AgGGPPS, CfTPS2, CfTPS3	13R-manoyl oxide	10 mg/l	Nielsen et al. (2014)
Synechocystis	MEP	CfTPS2, CfTPS3	13R-manoyl oxide	0.45 mg/g DCW	Englund et al. (2015)
S. cerevisiae	MVA	CcCLS, SpMiLS, PtAO	3β-OH-manoyl oxide	4.87 mg/l	Ignea et al. (2015)
S. cerevisiae	MVA	CcCLS, SpMiLS	13R-manoyl oxide	80 mg/l	Ignea, Athanasakoglou, et al. (2016a), Ignea, Ioannou, et al. (2016b)
S. cerevisiae	MVA	CcCLS, SpMiLS, SpCYP76AH24	11β-OH-manoyl oxide	21.9 mg/l	Ignea, Athanasakoglou, et al. (2016a), Ignea, Ioannou, et al. (2016b)
S. cerevisiae	MVA	CfTPS2, CfTPS3	13R-manoyl oxide	350 mg/l	Andersen-Ranberg et al. (2016)
S. cerevisiae	MVA	CfTPS2, CfTPS3, CfCYP76AH15, CfCYP76AH11, CfCYP76AH16, CfPOR, CfACT1–8	Forskolin	40 mg/l	Pateraki et al. (2017)
S. cerevisiae	MVA	CfTPS2, CfTPS3, CfCYP76AH15 (A99I) , CfCYP76AH11, CfCYP76AH16 , CfPOR, CfACT1–8	Forskolin	—	Forman et al. (2018)
S. cerevisiae	MVA	CfTPS2, CfTPS3, MET3p-ERG9	13R-manoyl oxide	167.1 mg/l	Guo et al. (2019)
S. cerevisiae	MVA	tCfTPS2, tCfTPS3, PHXT1-ERG9-PEST, BTS1∼GGGS∼ERG20 (F96C)	13R-manoyl oxide	3 g/l	Zhang et al. (2019)