Table 2.
Characteristics of human dorsal root ganglion neurons in media-only or coculture with Fadu cancer cells.
| Characteristic | Media only | Coculture | Significance |
|---|---|---|---|
| Cultured hDRG neurons | |||
| Percent with SA | 0% (0/10) | 8.3% (1/12) | n.s. (P = 0.35) |
| Percent with large DSFs | 0% (0/10) | 33.3% (4/12)* | P < 0.05 |
| AP amplitude (mV) | 120.8 ± 7.7 (10) | 115.2 ± 4.5 (12) | n.s. (P = 0.52) |
| AP rise time (ms) | 3.2 ± 0.7 (10) | 2.4 ± 0.6 (12) | n.s. (P = 0.39) |
| AP fall time (ms) | 16.5 ± 5.8 (10) | 10.0 ± 1.7 (12) | n.s. (P = 0.26) |
| Width at 0 mV (ms) | 9.6 ± 3.2 (8) | 4.2 ± 0.8 (11) | n.s. (P = 0.08) |
| AP overshoot (mV) | 58.7 ± 5.7 (8) | 61.2 ± 3.0 (11) | n.s. (P = 0.68) |
| AP afterhyperpolarization (mV) | 6.2 ± 2.9 (3) | 18.6 ± 2.9 (6) | n.s. (P = 0.24) |
| Threshold potential (mV) | −6.3 ± 4.9 (10) | −14.2 ± 3.6 (12) | n.s. (P = 0.20) |
Data expressed as percentages or mean ± SEM with the number of neurons in parentheses.
*
P < 0.05.
AP, action potential; DRG, dorsal root ganglion; DSF, depolarizing spontaneous fluctuations; .DSF, depolarizing spontaneous fluctuation; SA, spontaneous activity.