Fig. 1.

Threshold determination by BFF. BFF places count thresholds at the minimum density between fitted peaks of log-scale raw count distributions. To find thresholds, BFF first fits log-scale raw count distributions with a smoothed distribution model obtained using Kernel Density Estimation (KDE). Thresholds are then placed at the minimum density values between the peaks of the model. The data shown are histograms where the y-axis displays the square-root of density for each barcode in the Barnyard Dataset dataset. The colored curves represent the model fits of the data. These same colors are used throughout the article when displaying log-scale barcode count data