TABLE 2.

Quantification of m-xylene consumption and sulfide formation by strain mXyS1

Expa	Amt (mmol) of:
	m-Xylene added	m-Xylene consumedb	Sulfide producedc	Electrons from m-xylene consumedd	Electrons consumed by SO42− reductione
Strain mXyS1 with small amount of m-xylene	0.242	0.231	1.07	9.7	8.56
Strain mXyS1 with large amount of m-xylene	1.21	1.11	5.0	46.6	40.0
Strain mXyS1 without m-xylene (control)	0.0	0.0	0.067	0.0	0.54
Sterile medium without cells (control)	1.21	0.0	0.0	0.0	0.0

^aExperiments were carried out under anoxic conditions with flat bottles (250 ml) with a culture volume of 190 ml. The total amount of sulfate added to each bottle was 5.23 mmol (27.5 mM). The medium was overlaid with 5 ml of heptamethylnonane as the carrier phase for m-xylene. The volume of m-xylene to be added was calculated from the density (0.866 g · cm⁻³ at 20°C) and molecular mass (106.2 g · mol⁻¹). 

^bDifference between m-xylene added and m-xylene recovered in the carrier and aqueous phase at the end of the experiment. 

^cThe small amount of sulfide produced in the control with cells without substrate was subtracted from the amount of sulfide produced in experiments with cells and m-xylene. 

^dStoichiometrically, 42 mmol of electrons is derived from 1 mmol of m-xylene oxidized to CO₂. 

^eStoichiometrically, 8 mmol of electrons is required for complete reduction of 1 mmol of SO₄²⁻ to 1 mmol of H₂S.