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. 2022 May 5;18(5):e1010471. doi: 10.1371/journal.ppat.1010471

Fig 3. Phagocytic cells are required for HSV-2-induced immunopathology in Ifnar-/- mice.

Fig 3

(A) Total number of CD45+F480+ cells in vaginal tissue following infection (n = 5). (B) %CD45+F480+ to total CD45+ cells in vaginal tissue of WT and Ifnar-/- mice at 0 and 3 dpi following HSV-2 infection (n = 5). (C) Schematic of Ifnar-/- mice and NRG mice receiving clodronate or PBS control liposomes. (D) H&E staining of vaginal cross-sections of HSV-2-infected WT and Ifnar-/- mice administered PBS or clodronate liposomes at 3 dpi (n = 5–6). (E) Pathological score of (D). (F) Viral titers of vaginal washes at 1 and 3 dpi of HSV-2-infected Ifnar-/- mice given PBS or clodronate liposomes (n = 4–5). (G) H&E staining of vaginal cross-sections of HSV-2-infected NRG + α-IFNAR Ab mice with PBS or clodronate liposomes at 3 dpi (n = 4–5). (H) Pathology score of (G). 10x scale bar represents 200 μm, 20x scale bar represents 100 μm. Data in (A), (B), (E), (F), (H) and are represented as mean ± SEM. *p < 0.05, and ****p < 0.0001 (A, B, F, two-way ANOVA; E, one-way ANOVA; H, two-tailed t-test). See also S3 Fig.