Figure 2.

Effects of acetaminophen (APAP) on germ cells. Percentage (A) and number (B) of M2A-positive germ cells was assessed by flow cytometry following 7 days of exposure to either vehicle (–, white bar) or APAP 10⁻⁸ to 10⁻³M (gray to black bars). Total M2A-germ cells number per ovary is expressed as 10³ germ cells per ovary at 7, 8 to 9, and 10 to 12 DW. Data are represented as mean ± SE of the mean. Mixed linear models with fetus as a random effect were performed for cell numbers, densities, and percentages. *P < 0.05; **P < 0.01; ***P < 0.001, from the t-test comparisons with the control condition. The number of samples tested per condition is indicated at the bottom of each bar.