Figure 6.

Effects of acetaminophen (APAP) on human fetal ovarian sex steroids production. (A and B) Amounts of progesterone, pregnenolone, 17 hydroxyprogesterone (17OHP), and estradiol (E2) produced by APAP-exposed explants, normalized with amounts produced by control explants, after 7 days of exposure. Data from 8- to 9-DW (A) and 10- to 12-DW ovarian explants (B) are shown for normalized values of APAP at 10⁻⁶ to 10⁻³M (gray to black bars). Data are represented as mean ± SE of the mean. **P-value < 0.01, Wilcoxon post hoc test compared to control. The number of individuals per condition is indicated in the exposure key. (C-E) Representative in situ hybridization of nuclear estrogen receptors ESR1 (C), ESR2 (D), and the membrane estrogen receptor GPER messenger RNAs (E) in a 13.6-DW human fetal ovary. Lower panel photos show higher magnifications. Scale bar: 100 µm.