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Effects of kisspeptin on alkaline phosphatase (ALP) activity in human mesenchymal stem cells. Kisspeptin 1 nM significantly increased ALP activity during osteoblastogenic differentiation of hMSC-TERT4 cells. Presented as mean, each dot indicating a technical replicate, showing an increase in ALP activity at 1 nM compared with control (0) of 41.1% (**P = .0022). Repeated measures 1-way ANOVA with Dunnett’s multiple comparisons test, n = 6.
