Figure 3.

The p300 inhibitor A-485 exerts antitumor effects in vitro. (a) The IC₅₀ of A-485 in GH3 cells was detected after 3 days of A-485 incubation. (b) The cell viability was detected at 24, 48, 72, and 96 hours, respectively. (c) The protein expression of H3K18ac and H3K27ac in A-485 treated GH3 cells. (d) Apoptosis analysis of A-485 treated GH3 cells. (e) Cell cycle analysis of A-485 treated GH3 cells. (f) Clone formation of A-485 treated GH3 cells. (g) The mRNA expression of Gh1 in A-485 treated GH3 cells. (h) The secretion of GH in the supernatant of A-485 treated GH3 cells. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. The bar represents ± SD.