Fig. 3. Radiation-guided delivery of anti-MGMT oligonucleotides (AMONs) to reduce MGMT protein expression in D283 medulloblastoma orthotropic brain tumor model.

Athymic nude rats were inoculated with MGMT expressing D283 medulloblastoma cells (intra-cerebellar, n = 14). Rats received a single dose of 2 Gy cranial irradiation. AMON (10.5 mg/kg; IV) was administered 1 day after radiation to half (n = 7) of animals and all tumors were harvested 3 days later. A Immunoblot of MGMT, bcl-XL (apoptosis marker), and p27 (cell-cycle biomarker to confirm radiation delivery) of rat cerebellar tumors; Tubulin level was used as a total protein loading control. B–D Semi-quantification of MGMT, bcl-XL, and p27 immunoblotting signals. Data were presented as mean ± SEM. Statistical significance indicated by *P < 0.05. **P < 0.01.