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. 2021 Sep 21;29(5):445–455. doi: 10.1038/s41417-021-00387-5

Fig. 2. Overexpression of LIN28B enhances cell proliferation and induces tumour initiation capacity.

Fig. 2

MTT assays were used to measure cell viability on days 0, 1, 3 and 5 in the control and LIN28B-overexpressing MMNK-1 cell lines (a). The gene expression levels of the CCND1, CDC25A and CDK6 genes were assessed by RT-qPCR (b). Representative images of the soft agar colony formation assay of LIN28B-overexpressing MMNK-1 cells compared to the control (c). Total colonies from nine focal planes of the acquired images were counted, and their size was measured (d). The sphere-forming capability of LIN28B-overexpressing MMNK-1 cells is shown in e, spheroids were counted, and the size was measured (f). Representative tumours from control and LIN28B-overexpressing MMNK-1 xenograft mice (n = 4 in each group) (g) and tumour volume at day 28 of the experiment (h). Data are presented as the mean ± SD, n = 3 and mean ± SEM for h. Statistical significance is indicated by *P < 0.05, **P < 0.01, ***P < 0.001.