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. 2021 Sep 21;29(5):445–455. doi: 10.1038/s41417-021-00387-5

Fig. 4. LIN28B enhances components of the TGF-β signalling pathway in MMNK-1 cells.

Fig. 4

The volcano plot represents the identified proteins and is categorised into upregulated and downregulated expression according to fold change when normalised to control cells (a). A false discovery rate (FDR) of 0.05 was used as a cut-off value to consider significant differentially expressed proteins. The expression levels of TGFBI and TGFBRI proteins in control and LIN28B-overexpressing MMNK-1 cells were measured by western blot (b). The amount of secreted TGF-β complex in culture medium was measured using Bio-Plex Pro™ TGF-β assays (c). TGFBI expression levels in LIN28B-overexpressing MMNK-1 cells treated with the TGFBRI inhibitor SB431542 (10 µM) for 24 h were determined by western blot (d). The expression of the indicated genes in LIN28B-overexpressing MMNK-1-treated cells was determined using RT-qPCR, and the data are shown as relative expression to nontreated cells (e). The cell migration ability of LIN28B-overexpressing MMNK-1 cells treated with SB431542 was determined by wound healing assay (f), and quantitative wound closure was demonstrated by the percentage of wound confluence (g). Data are shown as the mean ± SD, n = 3. Statistical significance is indicated by *P < 0.05, **P < 0.01, ***P < 0.001.