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. 2021 Sep 21;29(5):445–455. doi: 10.1038/s41417-021-00387-5

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — Quantitative LIN28B gene expression in two clones of LIN28B-overexpressing CCA cells, #1 and #2, was determined by RT-qPCR (a). Data are shown as the fold change of gene expression normalised to cells containing an empty vector control. A cell scratching assay was performed to determine the capability of cells to close the wound gap. Representative images of wound closure of control cells and two clones of LIN28B-overexpressing cells after 6 h and 12 h incubation are shown (b). The capability of LIN28B-induced cell migration in KKU-214 and HuCCT-1 cells is shown by the percentage of wound area normalised to the initial wound gap (0 h) (b, c). The expression of EMT-related genes in LIN28B-overexpressing KKU-214 and HuCCT-1 cells was analysed by RT-qPCR (d, e). The total number and average size of counting spheroids in control cells and LIN28B-overexpressing KKU-214 (f) and HuCCT-1 cells (g) are shown as the mean ± SD of triplicate wells. Statistical significance is indicated by **P < 0.01, ***P < 0.001. ns is noted as no statistically significant.