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. 2022 May 18;43(3):301–318. doi: 10.24272/j.issn.2095-8137.2021.397

Figure 2.

Figure 2

HPPC induced autophagy of circulating hemocytes (blood cells) in early and mid-stage period after modeling

MDC and Lyso-Tracker Red staining marked autophagic hemocytes in hemolymph at 48, 96, and 192 h after modeling. Positive rate (%)=(fluorescent cell number/total cell number)×100. Scale bars: 100 μm. A: Fluorescence image of MDC-stained hemocytes 96 h after modeling (n=3). B: MDC staining positive rate in hemocytes. Significant differences between groups are indicated with different letters (P≤0.05, n=3). C: MDC staining positive rate in different types of hemocytes. D: Lyso-Tracker Red staining fluorescence image of hemocytes 48 h after modeling. E: Lyso-Tracker Red staining positive rate of hemocytes (n=3). F, G: Transcription levels of autophagy genes (F) Atg6 and (G) Atg8 in hemocytes analyzed by qPCR (n=3). Internal reference gene was Bombyx mori Rp49. Data are mean±SEM. ns: P>0.05;*: P<0.05;**: P<0.01;***: P<0.001, Student’st-test.