TABLE 1.
Purification of peroxidase from supernatant of G. candidum Dec 1 culture
| Purification step | Protein (mg) | Total activity (U) | Sp act (U/mg) | Activity yield (%) | Purification (fold) |
|---|---|---|---|---|---|
| Supernatant | 88 | 24a | 0.28a | 1 | |
| Ultrafiltration (YM 10) | 28 | 4.2 × 102 | 15 | 100a | 55 |
| Super Q chromatography | 7.8 | 3.0 × 102 | 34 | 71 | 140 |
| Butyl Toyopearl chromatography | 1.5 | 85 | 57 | 20 | 210 |
a
Total and specific activities were underestimated, presumably because of the presence of inhibitors in the supernatant; thus, the activity yield was calculated based on the value after YM 10 treatment.