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Kinetic parameters for activities of DyP and HRPa
| Parameter | Unit(s) | Value
|
|
|---|---|---|---|
| DyP | HRP | ||
| Km(RB5) | μM | 54 | 58 |
| Km(H2O2) | μM | 26 | 36 |
| Kcat(obs) | s−1 | 260 | 140 |
| Kcat/Km(RB5) | M−1 s−1 | 4.8 × 106 | 2.4 × 106 |
| Kcat/Km(H2O2) | M−1 s−1 | 1.0 × 107 | 3.8 × 106 |
RB5 decolorization by DyP and HRP was determined by pseudo-first-order kinetics.
