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. 2022 May 17;12:8126. doi: 10.1038/s41598-022-11986-z

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Characteristics of adipose tissue-derived multi-lineage progenitor cells (ADMPCs). (a) Mesenchymal stem cell (MSC) surface marker expression on ADMPCs. The expression of each surface marker is shown on the shaded histogram. Staining with an isotype control mAb is represented by a black line. (b) Colony formation of ADMPCs. Cells were seeded with the indicated density and cultured for 2 weeks. Cells were then fixed and stained with 0.1% crystal violet. (c) Multipotency of ADMPCs. Representative images are shown of Alizarin red staining of ADMPCs cultured in osteogenic differentiation media for 28 days, Oil red O staining of ADMPCs cultured in adipogenic differentiation media for 24 days, and Toluidine blue staining of ADMPCs cultured in chondrogenic differentiation media for 20 days.