Fig. 4.
RNA-dependent and independent nuclear protein structure and effects of GFP-tags on SAF-A binding. For all images: scale bars 5 μm. Cell types: normal human fibroblasts (Tig-1) & immortalized human female kidney cells (Hek293). A SAF-A and Pan-RNP proteins are released when C0T-1 RNA is digested with RNase in normal fibroblast cells, while NuMa, MATR3, Lamin A/C, and Lamin B1 are not. Outline of nuclei (delineated by DAPI DNA) in white for all but LaminB1. B Endogenous full-length SAF-A releases from chromosomes during normal mitosis in Hek293 cells. Color channels are separated to the right. C In contrast, when tagged with GFP, the normal full-length SAF-A protein remains bound to mitotic chromosomes throughout mitosis. Note enrichment of SAF-A on both inactive X-chromosomes in Hek293 prophase cell