Table 3.
LncRNA studies associated with in vitro functional analyses for livestock species
| lncRNA name | lncRNA impact | Cellular model | Strategy | Year (Refs) |
|---|---|---|---|---|
| A. Chicken | ||||
| MHM |
Embryonic development Sex determination |
Egg (0-day blastoderms) | OverEx | 2012 (Roeszler et al. 2012) |
| B. Cow | ||||
| ADNCR | Impact on SIRT1 by competing with miR-204 as a ceRNA to regulate adipogenesis | HEK293T,HEK293A & ADSC cells |
OverEx KD by siRNA |
2016 (Li et al. 2016) |
| LncRNA candidate 1 | Embryonic developmental rates | Cattle matured oocytes | KD by siRNA | 2015 (Caballero et al. 2014) |
| H19 | Differentiation of satellite cells. Blocking of the Sirt1/FoxO1 pathway during myogenesis | C2C12 cells & satellite cells (from adult cattle muscle) |
OverEx KD by pLenti-NTC interference vector |
2017 (Xu et al. 2017) |
| lnc403 |
Inhibit myogenic differentiation of bovine skeletal muscle satellite cells Negatively regulated gene Myf6 and positively regulated protein KRAS |
Satellite cells (from foetal bovine muscle) |
OverEx KD by siRNA |
2020 (Zhang et al. 2020) |
| IGF2 AS | Promote proliferation and differentiation of bovine myoblasts through various pathways | Myoblasts (from foetal bovine muscle) |
OverEx KD by siRNA |
2020 (Song et al. 2020) |
| C. Pig | ||||
| lncIMF4 | Associated with adipogenesis and effect in intramuscular preadipocyte proliferation and differentiation | Intramuscular preadipocytes (from 2 pig breeds) | KD by siRNA | 2020 (Sun et al. 2020) |
| TCONS_00815878 | Decreasing of Myod, MyoG and MyHC such as glycolysis and pyruvate metabolism which are related to skeletal muscle satellite cell differentiation | Skeletal muscle satellite cells | KD by ASO | 2019 (Huang et al. 2019) |
| XLOC-2222497 | Regulate AKR1C1 and progesterone metabolism | Endometrial cells |
OverEx KD by ASO |
2020 (Su et al. 2020) |
KD knock-down, OverEx overexpression