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. 2022 Feb 3;30(1):103–115. doi: 10.1007/s40199-022-00436-w

Table 1.

Nucleotide sequence and annealing temperature for used primers in qPCR

Target Gene Primer Sequence (5′-3′) Accession number AT (°C)
Cacpase-3 FWD: AGAACTGGACTGTGGCATTGAG NC_000004.12 59
REV: GCTTGTCGGCATACTGTTTCAG
MMP-9 FWD: GATGCGTGGAGAGTCGAAA NC_000020.11 59
REV: TAGGTGATGTTGTGGTGGTG
β-actin FWD: CTTCTACAATGAGCTGCGTG NC_000007.14 50
REV: CATGAGGTAGTCAGTCAGG