Fig. 3.

Liver H&E staining analysis of largemouth bass fed different experimental diets for 8 weeks. C0, C25, C50, C75, and C100 represent diets in which Chlorella meal replaced fish meal levels of 0%, 25%, 50%, 75%, and 100%, respectively. (A) Representative images (100×, scale bar = 100 μm); The black arrows represent the cell nucleus disappear and the cytoplasmic vacuolization. (B) Images' statistical analysis (mean ± SEM, n = 9); Liver relative health status of largemouth bass was scored according to the degree of its vacuolization (1 to 2: severe damage, 3 to 4: moderate, 5 to 6: mild, 7 to 8: slight, 9 to 10: normal). Duncan's multiple range test used to detect its significance. Values marked with different superscripts above the bars indicate significant differences (P < 0.05).