Fig. 4.

Liver DAPI and TUNEL staining analyses of largemouth bass fed different experimental diets for 8 weeks. C0, C25, C50, C75, and C100 represent diets in which Chlorella meal replaced fish meal levels of 0%, 25%, 50%, 75%, and 100%, respectively. (A) Representative images (200 × , scale bar = 100 μm). Under the fluorescence microscope, the blue color represents the nucleus and the green color represents apoptotic cells. (B) Images' statistical analysis (mean ± SEM, n = 9). Apoptosis rate (%) = 100 × [Green color area (TUNEL area)]/[Bule color area (DAPI area)]. TUNEL = terminal deoxynucleotidyl transferase dUTP nick end labeling; DAPI = 40, 6-diamidino-2-phenylindole. Duncan's multiple range test used to detect its significance. Values marked with different superscripts above the bars indicate significant differences (P < 0.05).