Table 1.
Formulation and chemical composition of the reference diet (% dry matter).
| Item | Content |
|---|---|
| Ingredients | |
| Fish meal1 | 42 |
| Gluten2 | 20 |
| Cassava starch3 | 15 |
| Fish oil4 | 5 |
| Vitamin and mineral additives5 | 1.4 |
| Monocalcium phosphate6 | 2 |
| Choline chloride7 | 2 |
| Guar gum8 | 1.5 |
| Microcrystalline cellulose9 | 11 |
| Y2O310 | 0.1 |
| Total | 100 |
| Proximate composition | |
| Moisture, % wet weight | 4.76 |
| Crude protein | 44.31 |
Fish meal: from Superprime, TASA Fish Product Co., Ltd., Peru.
Gluten: from Henan Midaner Trading Co., Ltd., Xinzheng, Henan, China.
Cassava starch: from Wuhan Yiteng Starch Co., Ltd., Wuhan, Hubei, China.
Fish oil: from Coland Feed Co., Ltd., Wuhan, Hubei, China.
Vitamin and mineral additives: from Guangdong Nutriera Group, Guangzhou, China. Vitamin additives, mg/kg diet: vitamin A 10; vitamin B1 6; vitamin B2 5; vitamin B6 7.5; vitamin B12 (1%) 4; niacinamide 50; ascorbyl calcium phosphate (35%) 500; calcium pantothenate 20; biotin (2%) 2.5; folic acid 5; vitamin E (50%) 200; vitamin K3 10; vitamin D3 5; inositol 100; corn protein powder 75. Mineral additives, mg/kg diet: CuSO4·5H2O 10; FeSO4·H2O 300; ZnSO4·H2O 200; MnSO4·H2O 100; KIO3 (10%) 80; Na2SeO3 (10% Se) 67; CoCl2·6H2O (10% Co) 5; NaCl 100; zeolite 638.
Monocalcium phosphate: from Sinopharm Chemical Reagent Co., Ltd., China.
Choline chloride: from Guangdong Nutriera Group, Guangzhou, China.
Guar gum: from Henan Wanbang Chemical Technology Co., Ltd., China.
Microcrystalline cellulose: from Shandong Liujia Pharmaceutical Excipients Co., Ltd., Jining, Shandong, China.
Y2O3: from Sinopharm Chemical Reagent Co., Ltd., China.