Table 6.
Growth, feed utilization, and morphological indices of largemouth bass fed different experimental diets for 8 weeks (mean ± SEM, n = 3 for IBW, FBW, WGR, SGR, FI, FCR, and SR; n = 9 for HSI and CF).
| Item | Diets1 |
Pr > F2 |
||||||
|---|---|---|---|---|---|---|---|---|
| C0 | C25 | C50 | C75 | C100 | ANOVA | Linear trend | Quadratic trend | |
| IBW3, g/fish | 17.65 ± 0.08 | 17.68 ± 0.07 | 17.60 ± 0.09 | 17.68 ± 0.09 | 17.57 ± 0.07 | 0.804 | 0.474 | 0.718 |
| FBW4, g/fish | 66.85 ± 2.03bc | 71.70 ± 2.11c | 68.93 ± 1.43bc | 64.83 ± 2.14b | 51.21 ± 1.97a | 0.000 | 0.003 | 0.000 |
| WGR5, % | 278.80 ± 13.09bc | 305.56 ± 10.47c | 291.77 ± 9.43bc | 266.64 ± 10.62b | 191.54 ± 11.48a | 0.000 | 0.003 | 0.000 |
| SGR6, %/d | 2.22 ± 0.06b | 2.33 ± 0.04b | 2.27 ± 0.04b | 2.16 ± 0.05b | 1.78 ± 0.06a | 0.000 | 0.003 | 0.000 |
| FI7, g/fish | 41.39 ± 1.24b | 45.22 ± 1.43b | 44.49 ± 0.79b | 43.49 ± 1.63b | 36.58 ± 1.79a | 0.010 | 0.106 | 0.001 |
| FCR8 | 0.84 ± 0.01a | 0.84 ± 0.01a | 0.87 ± 0.01a | 0.92 ± 0.01b | 1.09 ± 0.01c | 0.000 | 0.000 | 0.000 |
| SR9, % | 91.67 ± 4.41 | 96.67 ± 3.33 | 98.33 ± 1.67 | 96.67 ± 1.67 | 96.67 ± 1.67 | 0.540 | 0.259 | 0.226 |
| HSI10, % | 3.02 ± 0.11c | 3.37 ± 0.12c | 3.54 ± 0.10c | 4.32 ± 0.27b | 4.94 ± 0.23a | 0.000 | 0.000 | 0.000 |
| CF11, g/cm3 | 2.32 ± 0.05 | 2.24 ± 0.02 | 2.28 ± 0.03 | 2.29 ± 0.03 | 2.22 ± 0.05 | 0.174 | 0.238 | 0.496 |
| Quadratic regressions | R2 | Ymax12, % | Replace fish meal level13, % | |||||
| YFBW = - 0.0002X2 + 0.0601X + 66.8621 | 0.8577 | 15.03 | 31.7 | |||||
| YWGR = - 0.0011X2 + 0.338X + 278.8271 | 0.8576 | 15.43 | 32.6 | |||||
| YSGR = - 5E-06X2 + 0.0016X + 2.2125 | 0.8633 | 15.03 | 31.7 | |||||
C0, C25, C50, C75, and C100 represent diets in which Chlorella meal replaced fish meal levels of 0%, 25%, 50%, 75%, and 100%, respectively. Duncan's multiple range test was used to detect the significance of differences among all groups. Different superscripts on the same row indicate significant differences (P < 0.05).
Significance probability associated with the F-statistic.
IBW = initial body weight.
FBW = final body weight.
Weight gain rate (WGR) = 100 × (Final body weight- Initial body weight)/Initial body weight).
Specific growth rate (SGR) = 100 × [ln (Final body weight) − ln (Initial body weight)]/Days.
FI = feed intake (total amount of the dry feed consumed).
Feed conversion ratio (FCR) = Feed intake/(Total final body weight – Total initial body weight).
Survival rate (SR) = 100 × (Final fish number/Initial fish number).
Hepatosomatic index (HIS) = 100 × (Liver weight/Whole body weight).
Condition factor (CF) = 100 × (Body weight/Body length3).
Based on the quadratic regression analysis between the parameters and dietary Chlorella meal level to calculate the optimal Chlorella meal content.
Based on the Ymax to calculate the replace fish meal level by Chlorella meal.