REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Goat Anti-JAM-A	R&D Systems	AF1077; RRID: AB_2100596
Mouse Anti-JAM-A (Clone: J10.4)	In-House Reagent	Commercially Available; sc-53623; RRID: AB_784134
Rabbit Anti-JAM-A	Invitrogen	36-1700; RRID: AB_2533241
Rabbit Anti-p-YAP S127	Cell Signaling Technology	4911; RRID: AB_2218913
Rabbit Anti-YAP1	Cell Signaling Technology	14074; RRID: AB_2650491
Rabbit Anti-Ki67	Abcam	ab15580; RRID: AB_443209
Mouse Anti-ZO-1	Thermo Fisher Scientific	33-9100; RRID: AB_2533147
Rabbit Anti-EVI1	Thermo Fisher Scientific	711037; RRID: AB_2633107
Rabbit Anti-LATS1	Cell Signaling Technology	3477; RRID: AB_2133513
Rabbit Anti-p-LATS1 S909	Cell Signaling Technology	9157; RRID: AB_2133515
Rabbit Anti-NF2	Cell Signaling Technology	6995; RRID: AB_10828709
Rabbit Anti-NF2	Cell Signaling Technology	12888; RRID: AB_2650551
Rabbit Anti-p-NF2/Merlin S518	Cell Signaling Technology	9163; RRID: AB_2149793
Rabbit Anti-Calnexin	Sigma-Aldrich	C4731; RRID: AB_476845
Mouse Anti-14-3-3	BD Bioscience	561465; RRID: AB_10896967
Rabbit Anti-GAPDH	Sigma-Aldrich	G9545; RRID: AB_796208
Rabbit Anti- ZO-2	Life Technologies	389100; RRID: AB_2533390
Mouse Anti-ZO-2	Thermo Fisher Scientific	37-4700; RRID: AB_2533321
Mouse Anti-HA	Sigma-Aldrich	H9658; RRID: AB_260092
Rabbit Anti-HA	Sigma-Aldrich	H6908; RRID: AB_260070
Rabbit Anti-pan-TEAD	Cell Signaling	13295; RRID: AB_2687902
Chemicals, peptides, and recombinant proteins
FuGENE 6	Promega	E2691
Lipofectamine™ 3000	Thermo Fisher Scientific	L3000015
Lipofectamine™ RNAiMAX reagent	Thermo Fisher Scientific	13778075
Verteporfin	Sigma-Aldrich	SML0534
Tamoxifen	Sigma-Aldrich	T5648
Corn oil	Sigma-Aldrich	C8267
Matrigel	Corning	365237; Lot 9112015
Recombinant human EGF	R&D Systems	236-EG
Antibiotics/antimycotic	Corning	30-003-Cl
(Ζ)-4-hydroxytamoxifen	Sigma-Aldrich	H7904
Protein A agarose beads	Thermo Fisher Scientific	20333
Protein G agarose beads	Thermo Fisher Scientific	20398
Protein A Dynabeads	Thermo Fisher Scientific	1001D
Protein G Dynabeads	Thermo Fisher Scientific	1003D
NuPAGE LDS Sample Buffer	Thermo Fisher Scientific	NP0007
Prolong™ Gold anti-fade agent	Thermo Fisher Scientific	P36930
Antigen unmasking buffer	Vector Laboratories	H-3300-250
SsoAdvanced Universal SYBR Green Supermix	BioRad	1725270
Critical commercial assays
DC Protein Assay	Bio-Rad	5000111
Click-iT™ EdU Alexa Fluor™ 488 Imaging Kit	Invitrogen	C10337
Experimental models: Cell lines
SKCO-15	Gift from Enrique Rodriguez-Boulan, Sloan Kettering Institute	N/A
SW480	ATCC	CCL-228™
HEK293T	ATCC	CRL-3216™
Enteroids derived from JAM-AERΔIEC mice	This paper	N/A
Enteroids derived from JAM-A-/- mice	This paper	N/A
Enteroids derived from JAM-Af/f mice	This paper	N/A
Enteroids derived from C57BL/6 mice	This paper	N/A
Experimental models: Organisms/strains
C57BL/6 mice	Jackson Laboratory	000664; RRID: IMSR_JAX:000664
JAM-A−/− mice	Gift from T. Sato, Cornell University	N/A
JAM-Af/f mice	Flemming et al., 2018	N/A
Villin-CreERT2 mice	Gift from Sylvie Robine, Institut Curie-CNRS	N/A
JAM-AERΔIEC mice	This paper	N/A
Oligonucleotides
siRNA Targeting Sequence: JAM-A Target 1 GGGUGACCUUCUUGCCAAUU	This paper	N/A
siRNA Targeting Sequence: JAM-A Target 2 CGGGUGACCUUCUUGCCAAUU	This paper	N/A
siRNA Targeting Sequence: Evi1 Target 1 UCUAAGGCUGAACUAGCAGUUUU	Adapted from Huang et al. 2016	N/A
siRNA Targeting Sequence: Evi1 Target 2 GCUGAUUGCAGAACCCAAAUUUU	Adapted from Huang et al. 2016	N/A
siRNA Targeting Sequence: NF2 Target 1	Thermo Fisher Scientific	s224112
siRNA Targeting Sequence: NF2 Target 2	Thermo Fisher Scientific	s224112
siRNA Targeting Sequence: Non-Targeting Silencer™ Select Negative Control	Thermo Fisher Scientific	4390843
siRNA Targeting Sequence: TEAD1 Silencer™ Select	Thermo Fisher Scientific	4392420; s13962
siRNA Targeting Sequence: TEAD1 Silencer™ Select	Thermo Fisher Scientific	4392420; s13963
siRNA Targeting Sequence: TEAD2 Silencer™ Select	Thermo Fisher Scientific	4392420; s16075
siRNA Targeting Sequence: TEAD2 Silencer™ Select	Thermo Fisher Scientific	4292420; s16076
siRNA Targeting Sequence: TEAD3 Silencer™ Select	Thermo Fisher Scientific	4292420; s13967
siRNA Targeting Sequence: TEAD3 Silencer™ Select	Thermo Fisher Scientific	4292420; s13968
siRNA Targeting Sequence: TEAD4 Silencer™ Select	Thermo Fisher Scientific	4292420; s13964
siRNA Targeting Sequence: TEAD4 Silencer™ Select	Thermo Fisher Scientific	4292420; s13965
qPCR Primer Sequence Pair: EVI1 (human) Forward: GACCAAGTTTTTCCTGATTTGC Reverse: CCCTCTCTTCAGTATGTGACAGC	This paper	N/A
qPCR Primer Sequence Pair: Evi1 (mouse) Forward: TCCTTGAAGTGACTGCCATTC Reverse: ATGCGTACTTTACAGAGATCCG	This paper	N/A
qPCR Primer Sequence Pair: TBP (human) Forward: TGCACAGGAGCCAAGAGTGAA Reverse: CACATCACAGCTCCCCACCA	This paper	N/A
qPCR Primer Sequence Pair: Tbp (mouse) Forward: GGAATTGTACCGCAGCTTCAAA Reverse: GATGACTGCAGCAAATCGCTT	This paper	N/A
qPCR Primer Sequence Pair: β-actin (human) Forward: TCCCTGGAGAAGAGCTACGA Reverse: AGCACTGTGTTGGCGTACAG	This paper	N/A
qPCR Primer Sequence Pair: CTGF (human) Forward: CCTGGTCCAGACCACAGAGT Reverse: TGGAG ATTTTGGGAGTACGG	Fallah and Beaulieu, 2020	N/A
qPCR Primer Sequence Pair: CYR61 (human) Forward: TCCCTGTTTTTGGAATGGAG Reverse: GAGCACTGGGACCATGAAGT	Fallah and Beaulieu, 2020	N/A
Recombinant DNA
HA-tagged JAM-A pcDNA3.1 plasmid	Mandell et al. 2004	N/A
JAM-A-WT pcDNA3.1 plasmid	Mandell et al. 2004	N/A
JAM-A-DL1 pcDNA3.1 plasmid	Mandell et al. 2004	N/A
Software and algorithms
GraphPad Prism version 8.01	GraphPad Software, Inc.	https://www.graphpad.com/
ImageJ	NIH	https://imagej.nih.gov/ij/
Other
A1 Confocal Microscope	Nikon	N/A
Transwell filters	Costar	3450
Plastic Chamber Slides	Thermo Fisher Scientific	177445
Epoch microplate spectrophotometer	BioTek	N/A
RNeasy kit	Qiagen	74106