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. 2022 Apr 27;25(5):104317. doi: 10.1016/j.isci.2022.104317

Figure 2.

Figure 2

Enrichment of cells with spheroid forming ability by all four subpopulations

(A) Size of the four subpopulations spheroids from GBC02, SCC-070 CD24+ and SCC-032 cell lines.

(B) Second generation spheroid formation frequencies in limiting dilution assay from GBC02, SCC-070 CD24+ and SCC-032 cell lines.

(C) Estimation of SLCC frequencies in each subpopulation of (1) GBC02, (2) SCC-070 CD24+ and (3) SCC-032 cell lines using ELDA software.

(D) Frequencies of each subpopulation in colonies generated from Red, Orange, Green and Blue subpopulation specific single spheroids after 30 days (D-30) of sorting. Error bars represent mean ± SEM from four biological repeats and p values are denoted as: ∗∗∗∗p < 0.0001; ∗∗∗p < 0.001; ∗∗p < 0.01; ∗p < 0.05.