Figure 2.

Endothelial sprouting on substrates with different ligand lines after 24 h

(A–D) The top rows (A–C) show zoomed-in sections of representative samples with Jag1, Dll4, and Fc lines, highlighting the fluorescent beads (red) used in the μCP ink (column 2), and the actin skeleton (green) of the endothelial cells, shown as normal and inverted for clearer visual assessment (column 3 and 4, respectively). The bottom row (D) shows a representative image of a whole Jag1 sample, from which ROIs were taken for analysis. As expected, Fc lines did not seem to affect the sprouts, and sprouting did seem to be confined to space between Dll4 lines. However, Jag1 lines did not seem to affect endothelial sprouts, and a similar crossing behavior as in Fc samples could be observed. Scale bars in A–C represent 250 μm. Scale bar in D represents approximately 1.5 mm.