Fig. 11.

Comparison of otic placode/otocyst marker gene expression levels in otocyst cell clusters between RA-treated and untreated RA cells by scRNA-seq. (A) The dot plot shows the expression of selected otic placode/otocyst and otocyst-derived neuroblast and off-target lineage (mesenchyme, epidermal cells, neurons, anterior placode, epibranchial placode, cranial neural crest and peri-otic mesenchyme) marker genes. The spot size represents the percentage of cells expressing a selected gene. The blue and red spots indicate non-RA-treated cells (RA-) and RA-treated cells (RA+), respectively. (B) The bar plot shows the relative proportions of cells with and without RA treatment in each cluster. The blue and red bars indicate non-RA-treated cells (RA-) and RA-treated cells (RA+), respectively.