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. 2022 May 16;20:165–186. doi: 10.1016/j.reth.2022.04.008

Fig. 7.

Fig. 7

Immunocytochemical analysis of the posterior placodal and otic placodal cells induced from H9 hESCs. (A) Representative bright field images of floating spheres on Days 12, 14, 16 and 20. Scale bars, 100 μm. (B) Representative immunocytochemical images of induced cells on Days 12, 14, 16 and 20 of culture. The cells were coimmunostained for the otic placodal markers SIX1, PAX8 and SOX2. The nuclei were stained with Hoechst 33,342. Scale bars, Days 12, 14 and 16, 50 μm; Day 20, 100 μm.