Skip to main content
. 2022 Apr 29;61:101507. doi: 10.1016/j.molmet.2022.101507

Figure 4.

Figure 4

Knock-out of miR-29a in POMCCreCas9 mice leads to obesity and insulin resistance. (A–I) Phenotypic analyses of POMCCreCas9 females bilaterally injected by rAAVs equipped with sgRNAs targeting miR-29a/b-1 cluster with body macrophotographs (A), body weight (B) and Echo-MRI-based body composition analysis (C). For fat tissue, fat pad weights (D) were analyzed, macro- (E,F) and microphotographs of perigonadal (D-F,G) and perirenal (D–F) adipose tissue were taken. Food intake analysis (H) and glucose tolerance test 14 weeks after surgery (I). n = 5 and 6 for POMCCremiR-29aCKO and control mice, respectively). Error bars represent SEM. ∗, p < 0.05; ∗∗, p < 0.01; ∗∗∗, p < 0.001; ∗∗∗∗, p < 0.0001 as assessed by unpaired two-tailed Student's t-test (C,D) or 2-way ANOVA followed by post-hoc Holm–Sidak pairwise comparison tests (B,H,I). Scale bar (μm): 125.