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. 2022 May 6;15:100277. doi: 10.1016/j.mtbio.2022.100277

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© 2022 The Authors

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

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Fig. 3 — Effect of FPSOH hydrogel on anti-inflammation and macrophage polarization. (A) Expression of M1-ralated genes analyzed by RT-qPCR in RAW 264,7 macrophages. (B) Confocal images of M1 and M2 macrophages co-cultured with FPSOH. iNOS was stained for marking M1 macrophages. CD206 was stained for marking M2 macrophages. Scale bar is 50 μm. (C) and (D) Percentage of positive cells in confocal images of iNOS and CD206. (E) and (F) Flowcytometry analysis of macrophages treated by FSPOH in the presence of LPS plus IFN-γ for 24 h. (G) and (H) Western blotting displaying the expression of inflammatory-related proteins and the activation of NF-κB pathways with the indicated treatment. ∗P < 0.05; ∗∗P < 0.01; ∗∗∗P < 0.001.