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. 2022 May 4;13:893692. doi: 10.3389/fmicb.2022.893692

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Copyright © 2022 Rada, Hrdý, Zdrha, Narayanasamy, Smutná, Horáčková, Harant, Beneš, Ong, Tsai, Luo, Chiu, Tang and Tachezy.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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TVV transmission experiments. (A) Monitoring of TVVs by RT PCR in TV79-49c1^– clone after incubation with sEVs from TV79-49c1⁺. (B) RT PCR monitoring of TVVs, in TV79-49c1^– acceptor clone for 40 subcultures after co-cultivation with TVV79-49c1⁺ donor clone. TVV- plus clone was transformed with a plasmid for expression of puromycin N-acetyl transferase (PAC), and biotin ligase (BirA), and the TVV-minus clone was resistant to geneticin. The clones were mixed, co-cultivated for five subcultures, and then geneticin was added for 35 subcultures. (C) RT PCR monitoring of genes for BirA and PAC.