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. 2022 May 4;13:893692. doi: 10.3389/fmicb.2022.893692

FIGURE 4.

FIGURE 4

Comparison of sEVs proteomes from TV79-49c1– (TVV–) and TV79-49c1+(TVV +). (A) Volcano plot analysis of proteomes using statistical significance p-value 0.01. (B) Structured illumination microscopy of T. vaginalis TV79-49c1+ expressing HA-tagged BspA (TVAG_268070). Arrows indicate large vesicles reminiscent of multivesicular bodies. (C) Immunoblot detection of HA-tagged BspA (TVAG_268070) in cell lysate and sEVs from TV79-49c1+ transformed T. vaginalis. BspA corresponds to 87 kDa protein in the cell lysate and sEVs. An additional band of 50 kDa in the lysate is likely a product of BspA cleavage. D, E. RT PCR of selected genes for proteins, which were unique or upregulated in proteomes of TVV– (D) or TVV+ (E) sEVs. CBP, calcium-binding protein; ERGIC, endoplasmic reticulum-Golgi intermediate compartment protein; GP63, leishmanolysin-like metallopeptidase; hyp, conserved hypothetical protein; PK, CMGC family protein kinase; RIC2, repair of iron centers protein 2; TA, tyrosine aminotransferase. *p-value < 0.05, **p-value < 0.005, ***p-value = 0.0005.