TABLE 1.
Sequencing of RNAs isolated from sEVs that were shed by TV79-49c1–, and TV79-49c1+.
| Sample* | A: TVV– | A: TVV+ | B: TVV– | B: TVV+ | C: TVV– | C: TVV+ |
| Total read number (<15 bp) | 1,844,606 | 1,827,363 | 2,417,076 | 3,916,028 | 3,644,654 | 2,905,518 |
| Reference dataset TVV1-4** | 4 | 4 | 4 | 4 | 4 | 4 |
| Mapped reads TVV1, (% coverage) | 2 | 783 (45.7) | 0 | 2880 (53.6) | 0 | 1202 (47.4) |
| Mapped reads TVV2 (% coverage) | 4 | 248 (50.9) | 0 | 1480 (63.9) | 1 | 1059 (61.6) |
| Mapped reads TVV3 (% coverage) | 11 | 781 (56.3] | 1 | 6029 (86.2) | 0 | 3094 (82.1) |
| Mapped reads TVV4 | 0 | 1 | 0 | 1 | 0 | 0 |
| Reference transcript TV G3# | 98,611 | 98,611 | 98,611 | 98,611 | 98,611 | 98,611 |
| Total mapped reads(%)## | 1,046,850 (56,75) | 952,694 (52.13) | 1,310,931 (54.24) | 2,095,038 (53.5) | 1,910,888 (52.43) | 1,717,371 (59.11) |
| Unmapped (%) | 797,756 (43.25) | 874,669 (47.87) | 1,106,145 (45.76) | 1,820,990 (46.50) | 1,733,766 (47.57) | 1,188,147 (40.89) |
| tRNA RPM (%)† | 143,009 (14.3) | 415,034 (41.5) | 102,327 (10.23) | 161,381 (16.14) | 83,516 (8.35) | 232,954 (23.3) |
| rRNA RPM (%)† | 841 868 (84.19) | 558 347 (55.83) | 883 470 (88.35) | 814 944 (81.49) | 902,531 (90.25) | 748,027 (74.8) |
| Others RPM (%)† | 15,123 (1.51) | 26,619 (2.66) | 14,203 (1.42) | 23,675 (2.37) | 13,953 (1.4) | 19,019 (1.9) |
*RNA was isolated from three independent experiments (A, B, and C), each experiment included isolation of RNA cargo from TV79-49c1– (TVV–), and TV79-49c1+ (TVV+) sEVs.
**Reference dataset: TVV1 (NC_027701.1), TVV2 (NC_003873.1), TVV3 (NC_004034.1), and TVV4 (NC_038700.1).
#Reference genome: T. vaginalis G3 strain, TrichoDB (https://trichdb.org/trichdb/app).
##end-to-end (bowtie2, http://bowtie-bio.sourceforge.net/bowtie2/index.shtml).
†FeatureCounts has been used for assignment.
RPM, read per million, (%) percent of assigned reads.