Table 1.

AA methods

Method	Description
Radiobinding assay (RBA)	Radiolabeled antigens detected in antibody-antigen complexes
Electrochemiluminescence (ECL)	Biotin and sulfo-TAG–labeled ligands that emit light when activated
ELISA	Detection of antigen–antibody complexes by enzyme-linked reagents
Luciferase immunoprecipitation (LIPS)	Quantitates serum antibodies by measuring luminescence emitted by the reporter enzyme luciferase fused to an antigen of interest
Agglutination PCR (ADAP)	PCR amplification of DNA in DNA–antigen conjugates bound to antibodies to form aggregates

These methods are reviewed in So et al. (19).