Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 May 4;12:878920. doi: 10.3389/fonc.2022.878920

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2022 Vega, Nilsson, Kumar, Niitsu, Simmons, Ro, Wang, Chen, Joughin, Li, McKinley, Liu, Roland, Washington, Coffey, Lauffenburger and Lau

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Characterization of the squamous component present in AOM/DSS, but not APC, tumors. (A) Heatmap of genes related to WNT signaling and colonic stem cells. Columns represent single cells, arranged by epithelial cell type clusters. (B) Dot plot showing colonic or squamous identity genes for epithelial cell clusters, where clusters include cells from both APC tumor and AOM/DSS tumor samples. (C) Heatmap of fetal and embryonic programs. Columns represent single cells, arranged by epithelial cell type clusters. (D) UMAPs of scRNA-seq data with expression overlay, indicated by the color gradient, of selected fetal and embryonic genes. (E) Dot plot showing cell cycle meta-genes for each cell cycle phase in epithelial cell clusters. (F) CytoTRACE scores for epithelial cell types calculated from scRNA-seq data. (G) Representative H&E image of multiple AOM/DSS tumors within the same animals, with a focus on squamous components (insets). (H) Representative MxIF image of KRT14 in APC tumors and AOM/DSS tumors. Images were representative of n = 5 AOM/DSS tumors and n = 3 APC tumors. (I) Observation of Lrig1^CreERTe/+;Rosa^YFP/+ traced cells within a single squamous cell region of an AOM/DSS tumor.