Table 1.
Detection of honeybee viruses and 28s ribosomal RNA in V.orientalis by multiplex PCR.
| Apiary | Sample | DWV | SBV | ABPV | BQCV | KBV | CBPV | 28S |
|---|---|---|---|---|---|---|---|---|
| A | VO1 | + | – | + | + | – | – | + |
| VO2 | + | – | + | + | – | – | + | |
| VO3 | + | – | + | + | – | – | + | |
| VO4 | + | – | + | + | – | – | + | |
| VO5 | + | – | + | + | + | – | + | |
| VO6 | + | – | + | + | – | – | + | |
| VO7 | – | – | + | + | – | – | + | |
| VO8 | + | – | + | – | – | – | + | |
| VO9 | + | – | – | + | – | – | + | |
| VO10 | – | – | + | – | – | – | + | |
| B | VO11 | + | – | + | + | – | – | + |
| VO12 | + | – | + | + | – | – | + | |
| VO13 | – | – | – | – | – | – | + | |
| VO14 | + | – | + | – | – | – | + | |
| VO15 | + | – | – | – | – | – | + | |
| VO16 | – | – | – | + | – | – | + | |
| VO17 | + | – | + | – | – | – | + | |
| VO18 | + | – | + | – | – | – | + | |
| VO19 | + | – | + | – | – | – | + | |
| VO20 | + | + | + | – | – | – | + | |
| C | VO21 | + | – | – | – | – | – | + |
| VO22 | + | – | – | + | – | – | + | |
| VO23 | + | – | – | – | – | – | + | |
| VO24 | + | – | + | + | – | – | + | |
| VO25 | – | – | – | – | – | – | + | |
| VO26 | + | – | – | – | – | – | + | |
| VO27 | + | – | + | – | – | – | + | |
| VO28 | + | – | – | – | – | – | + | |
| VO29 | + | – | – | – | – | – | + | |
| VO30 | + | – | + | – | – | – | + |
Sampling apiary, sample names and viruses acronyms are indicated (DWV, Deformed Wing Virus; SBV, SacBrood Virus; ABPV, Acute Paralysis Virus; BQCV, Black Queen Cell Virus; KBV, Kashmir Bee Virus; Chronic Bee Paralysis Virus; + positive sample; - negative sample).