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. 2022 May 4;12:798767. doi: 10.3389/fcimb.2022.798767

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Copyright © 2022 Osman, Garrec, de Souza, Zarubica, Belhaouari, Baudoin, Lepidi, Mege, Malissen, Scola and Devaux

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Expression of E-cad and sE-cad. in Caco-2 cells. (A) Quantification of sE-cad (n=4) in the cell-culture supernatant of virus-free Caco-2 cells and cells exposed to coronaviruses SARS-CoV-2 or HCoV-OC43 at an MOI of 0.5 for 4 hours, 24 hours and 48 hours respectively, using antigen-specific ELISA. The results are the average of quadruplicates. (B) Western blot analysis of E-cad expression in virus-free Caco-2 cells and cells exposed for 48h to SARS-CoV-2 or HCoV-OC43 coronaviruses. GAPDH was used as loading control. (C) Quantitative representation of E-cad protein expression by Caco-2 cells infected or not with SARS-CoV-2 or HCoV-OC43 normalized with respect to GAPDH. The Image J program was used to quantify the Western blot bands intensity. The symbol ** means a p-value < 0.01. The symbol * means a p-value < 0.05.