Table 1.
Primers sequences used for the RT-qPCR.
| Genes | Symbols | Primers sequence | |
|---|---|---|---|
| Sense | Antisense | ||
| E-cadherin | CDH1 | 5’-GAAGGTGACAGAGCCTCTGGA T-3’ | 5’GATCGGTTACCGTGATCAAAA T-3’ |
| Angiotensin Conversion Enzyme 2 | ACE2 | 5’-CAGGGAACAGGTAGAGGACAT T-3’ | 5’CAGAGGGTGAACATACAGTTGG-3’ |
| Transmembrane Protease Serine 2 | TMPRSS2 | 5’-AAGTTCATGGGCAGCAAGTG-3’ | 5’-ACGCCATCACACCAGTTAGA-3’ |
| Transmembrane Protease Serine 4 | TMPRSS4 | 5’-CAAAGTAGAGGCAGGGGAAAA -3’ | 5’-CGGAAAAAGTTAGGACACAG GA-3’ |
| Neuropilin 1 | NRP-1 | 5’-GCTGGGAAGTGTGTTGATGAC-3’ | 5’ACAAAGGGGAGAGGAGAGAG AG-3’ |
| Sodium-dependent neutral amino acid transporter | B0AT1 | 5’-GGTGTGTGCCAGTATGATGTTC -3’ | 5’AAGAGCAGGAAAAGATGAGG TG-3’ |
| α-Disintegrin and metalloproteinase domain-containing protein 10 | ADAM-10 | 5’-AACCTACGAATGAAGAGGGAC A-3’ | 5’-TGACAGAGTGAAATGGCAGA GT-3’ |
| α-Disintegrin and metalloproteinase domain-containing protein 17 | ADAM-17 | 5’-GCAGGACTTCTTCACTGGACAC -3’ | 5’-TCTACTAACCCTTTTGGGAGC A-3’ |
| Angiotensin II Receptors 1 | AT1R | 5’-TGTGGACTGAACCGACTTTTCT -3’ | 5’-GGAACTCTCATCTCCTGTTGC T-3’ |
| Proto-oncogene Mas | MAS1 | 5’-GGAGAAAGAGACACCGCATAA C-3’ | 5’-GTGAAGAGACAGAGAACGA GCA-3’ |
| Proto-oncogene Mas Like | MAS1L | 5’-CTGCTCCTGACTGTGATGTTGT-3’ | 5’-GTTTGGGTTCTGTGCCTCCT-3’ |
| Housekeeping gene β-Actin | ACTB | 5’-CATGCCATCCTGCGTCTGGA-3’ | 5’-CCGTGGCCATCTCTTGCTCG-3’ |
| Platelet endothelial cell adhesion molecule | PECAM-1/CD31 | 5’-CAAAGACAACCCCACTGAAGAC-3’ | 5’-TCCAGACTCCACCACCTTACTT-3’ |
| Junctional adhesion molecule A/Platelet adhesion molecule 1 | F11R/JAM-A | 5’-GGATTTCTCAGGTCATTTGGAG-3’ | 5’-TAGACTGGTGGATGGTGGTAGA-3’ |
| Connexin-43 | Cx43 | 5’-GGTTCAAGCCTACTCAACTGCT-3’ | 5’-GTTTCTCTTCCTTTCGCATCAC-3’ |
| Occludin | OCLN | 5’-CTTCCATCCTGTGTTGACTTTG-3’ | 5’-CACTTTTCTGCCCTGATTCTTC-3’ |
| Zonulin | HP2 | 5’-ATGTGAAGCAGTATGTGGGAAG-3’ | 5’-AGAGATTTTTAGCCGTGGTCAG-3’ |
| Glyceradehyde-3-Phosphate dehydrogenase | GAPDH | 5’-ACACCCACTCCTCCACCTTT-3’ | 5’-CTCTTCCTCTTGTGCTCTTGCT-3’ |
| Beta-2 microglobulin | B2M | 5’-GGTTTCATCCATCCGACATT-3’ | 5’-GGCAGGCATACTCATCTTTTTC-3’ |