TABLE 1.

The amount of Cd²⁺ bound by envelopes of E. coli pop6510 expressing LamB-MBSs and a portion of Cd²⁺ removed from the surface by EDTA treatment^a

Plasmid transformed to E. coli pop6510	Amino acid sequences (MBSs) inserted into LamB proteinb	Cd2+ removed by EDTA treatment (%)c	Cd2+ bound by cell envelopes (nmol/mg [dry wt])d
pLBB9	None	69	125
pLBHP	N-Asp-Pro-Ala-Gly-His-His-Pro-His-Gly-Ala-C	60	196
pLBHP2	N-(Asp-Pro-Ala-Gly-His-His-Pro-His-Gly-Ala)2-C	59	226
pLBCP	N-Asp-Pro-Ala-Gly-Cys-Gly-Cys-Pro-Cys-Gly-Cys-Gly-Ala-C	55	227
pLBHPCP	N-Asp-Pro-Ala-Gly-Cys-Gly-Cys-Pro-Cys-Gly-Cys-Gly-Ala-Asp-Pro-Ala-Gly-His-His-Pro-His-Gly-Ala-C	51	184

^aAmino acid sequences corresponding to DNA fragments artificially introduced into lamB-153 (MBSs) and the names of the corresponding plasmids are indicated. 

^bThe amino acids of the MBSs are underlined. 

^cCells preadsorbed with cadmium as described in the legend to Fig. 3 were treated for 15 min with an excess volume of ice-cold 5 mM EDTA in 0.85% NaCl (pH 7.1), and the metal content was determined by atomic absorption spectrometry. The data represent the mean value of two independent experiments. 

^dThe mean value of two independent experiments with a span of less than 10%. First, 3 mg (dry wt) of the cell envelopes was incubated for 30 min in 3 ml of 5 mM CdCl₂ in 25 mM HEPES (pH 7.0). The metal content of the envelopes was then determined by atomic absorption spectrometry.