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. 2022 May 14;30:101278. doi: 10.1016/j.bbrep.2022.101278

Fig. 2.

Fig. 2

Differential scanning fluorescence (DSF) analysis in pH=7.4 PBS buffer of 2.5 μM h2E2 mAb previously treated for 30 min with TNM. Panel A – The first derivative of the fluorescence of the DASPMI reporter dye as a function of temperature, using 0–1000 μM of the three mAb ligands, cocaethylene (CE), cocaine (Coc), and benzoylecgonine (BE). Panel B – Plots of the Boltzmann melting temperature (TmB) from the raw fluorescence data used to generate the first derivative data in Panel A, as a function of ligand concentration. Panel C – The peak of the first derivative of the fluorescence data (TmD) shown in Panel A as a function of ligand concentration. Duplicate samples at each concentration of ligand were analyzed and shown in the figure.