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. 2022 Apr 6;20(7):1363–1372. doi: 10.1111/pbi.13813

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© 2022 The Authors. Plant Biotechnology Journal published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd.

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Observation of SARS‐CoV‐2 VLPs using transmission electron microscopy. (a) Purified fraction #6 from EV‐infiltrated leaves prepared by the same method as EMS and S. (b) Fraction #6 from leaves infiltrated with EMS. (c) Fraction #6 from leaves infiltrated with S. (d) image of VLPs in S sample at higher magnification (150 000×), with particles showing typical coronavirus morphology surrounded by dark blue boxes and an individual spike indicated by a red arrow. All samples were stained with 2% (w/v) uranyl acetate. Scale bars represent 100 nm.