Fig. 2.

Knockdown of circ_0090231 inhibited ox-LDL-mediated angiogenesis, apoptosis, oxidative stress, and inflammatory response in HUVECs. HUVECs were treated with control, ox-LDL, ox-LDL + sh-NC, and ox-LDL + shcirc_ 0090231. A) RT-qPCR assay was used to determine circ_0090231 level in treated HUVECs. B) Tube formation assay was applied to assess the number of capillary-like structures of HUVECs. C) Flow cytometry assay was performed to measure the apoptosis rate in treated HUVECs. *p < 0.05 D) Western blot assay was conducted to test the protein levels of Bcl-2 and Bax in treated HUVECs. E-G) The activities of MDA, LDH, and SOD were detected by commercially available kits in treated HUVECs. H-J) ELISA kits were used to examine the secretions of TNF-α, IL-1β, and IL-6 in treated HUVECs. *p < 0.05