Fig. 4.

Downregulation of miR-9-5p abolished the effects of circ_0090231 silencing on angiogenesis, apoptosis, oxidative stress, and inflammatory response in ox-LDL-treated HUVECs. A) miR-9-5p level was determined by RT-qPCR assay in HUVECs treated with control, ox-LDL, ox-LDL + miR-NC inhibitor, and ox-LDL + miR-9-5p inhibitor. B-J) HUVECs were treated with control, ox-LDL, ox-LDL + sh-NC, ox-LDL + sh-circ_0090231, ox-LDL + sh-circ_0090231 + miR-NC inhibitor, and ox-LDL + sh-circ_0090231 + miR-9-5p inhibitor. B) Tube formation assay presented the formation of capillary-like structures. C) Apoptosis rate of HUVECs was assessed by flow cytometry assay. *p < 0.05 D) Protein levels of Bcl-2 and Bax in HUVECs were measured by western blot assay. E-G) Special assay kits identified of MDA, LDH, and SOD products in treated HUVECs. *p < 0.05 H-J) The secretions of TNF-α, IL-1β, and IL-6 in treated HUVECs were detected by ELISA kits. *p < 0.05