TABLE 1.
Binding kinetics of wild‐type and RBD‐CBD variant with biotinylated ACE2
| RBD | Lineage name | Mutation on RBD | Binding affinity, K D (nM) | ka (1/Ms) | kdis (1/s) |
|---|---|---|---|---|---|
| WT | 12.7 | 99,200 | 0.00127 | ||
| Alpha | B.1.1.7 | N501Y | 8.17 | 122,000 | 0.000997 |
| Beta | B.1.351 | N501Y, K417N, E484K | 9.59 | 132,000 | 0.00126 |
| Gamma | P.1 | N501Y, K417T, E484K | 4.3 | 220,000 | 0.000954 |
| Delta | B.1.167.2 | L452R, T478K | 9.19 | 231,000 | 0.0021 |
| Kappa | B.1.167.1 | L452R, E484Q | 22.2 | 70,400 | 0.00156 |
| Epsilon | B.1.427/B.1.429 | L452R | 20.7 | 96,900 | 0.002 |
| Delta plus | AY.1 | L452R, T478K, K417N | 25.2 | 103,000 | 0.0026 |
| Eta | B.1.525 | E484K | 18.7 | 160,000 | 0.0029 |
| Lambda | C.37 | L452Q, F490S | 26.1 | 93,300 | 0.00244 |
| AD | N501Y, T478K | 3.01 | 153,000 | 0.000459 |
Abbreviations: ACE2, angiotensin‐converting enzyme II; AD, alpha–delta; CBD, cellulose binding domain; RBD, receptor binding domain.