Table 2.

Table of constructs prepared during the course of this study and their properties.

Name	Fusion (Amino Acids)	Myosin (Amino Acids)	Soluble?	Crystallized?	Diffraction?	Myosin Initial Amino Acid Coiled-coil Propensity *	Myosin Final Amino Acid Coiled-coil Propensity*
Gp7-MyH7-1660-1758-Eb1	Gp7 (2–49) Eb1 (214–256)	1660–1758	Yes	Yes	No visible diffraction	0.865	0.962
Gp7-MyH7-1677-1755-Eb1	Gp7 (2–49) Eb1 (207–256)	1677–1755	Yes	Yes	3.0 Å	1.000	0.986
Gp7-MyH7-1729-1786	Gp7 (2–49)	1729–1786	Yes	Yes	2.3 Å	0.854	0.996
Gp7-MyH7-1733-1797	Gp7 (2–49)	1733–1797	Yes	Yes	3.0 Å	0.943	0.998
Xrcc4-MyH7-1677-1754-Eb1	Xrcc4 (2–132) Eb1 (214–256)	1677–1754	Yes	Yes	<6 Å	1.000	0.990
Xrcc4-MyH7-1677-1758	Xrcc4 (2–132)	1677–1758	Yes	Yes	No visible diffraction	1.000	0.962
Xrcc4-MyH7-1677-1758	Xrcc4 (2–132)	1677–1758	Yes	Yes	3.5 Å	1.000	0.962
Xrcc4-MyH7-1678-1737	Xrcc4 (2–140)	1678–1737	Yes	No	n/a	1.000	0.982
Xrcc4-MyH7-1678-1751	Xrcc4 (2–140)	1678–1751	Yes	Yes	6 Å	1.000	0.997
Xrcc4-MyH7-1732-1790	Xrcc4 (2–137)	1732–1790	Yes	No	n/a	0.943	0.998
Xrcc4-MyH7-1733-1797	Xrcc4 (2–132)	1733–1797	Yes	Yes	2.5 Å	0.943	0.998

Names in Bold indicate structures determined during study.

^*Coiled-coil propensity was determined for cardiac myosin using a 14 amino acid window in the COILS server.