FIG. 1.
Physical map of plasmids pBKThb, pCKThb, pGPThb, pGDTh, and pGD71 constructed to express the thaumatin gene from efficient transcription initiation regions (thick arrows): PB2, 477 bp; PpcbC, 761 bp; PgpdA, 880 bp; PgdhA, 750 bp. The signal sequence and amino-terminal region of the B2 protein of A. chrysogenum (SS-B2) are indicated by shaded boxes. The KEX2 sequence is indicated as a black vertical thick line, and the synthetic gene of thaumatin II (tha) is shown by a thin arrow inside the open box. TCYC1, transcriptional terminator of S. cerevisiae. Vector pAN7-1 contains a hygromycin resistance marker, whereas pJL43 and pJL43b1 contain the phleomycin resistance marker. The following restriction sites were used: HindIII (H), BamHI (B), SalI (S), XbaI (X), and NcoI (N).